Coding

Part:BBa_K2927002

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-28)

10X His

This sequence encoded 10 connected histidines, which can be used for protein purification through strong interaction with Nickel (II) ion. It belongs to the compositions of Cas12a expression plasmid (10X His-MBP-TEV-Cas12a).

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None